AC Bio - Enzymes: Proteins that speed up reactions

Enzyme Lab Experiment Your apple company is in the business of making and selling apple juice. In order to increase profits the company would like to experiment with different enzymes to see if they can speed up juice production. Your job is to investigate the ability of two isolated enzymes for speed and cost efficiency. Enzyme information Two enzymes, cellulase and pectinase, are available to your company. We know that enzymes speed up reactions but what reaction are these two speeding up? Google what cellulase and pectinase do. Cellulase activity Pectinase activity Pectinase costs $50 and Cellulase costs $100 Based on the cost and the way the enzymes work will pectinase, cellulase, or a half-and-half mixture be best for your company and why? Setting up a plan Your teacher will help you answer these questions as teams in class Question Answer What is the problem you're trying to solve? What are you adding to the apple juice production in order to speed it up? This is known as our Independent Variable since that's what we're changing in the experiment. What are you going to measure as the outcome? This is our Dependent Variable since it's what we are measuring after our manipulation. Write a hypothesis using the following format: "If we add _ (put your independent variable in here) then we will be able to measure a change in _ (put your dependent variable in here) because _ (why do you expect this to happen)" What are you going to compare your results to? This is our control. In other words, how do we know the enzymes make any difference at all... are we spending money on nothing? What are three things that you should keep constant in your experiment? Write a clear procedure: Explain what materials you will use, how you'll set them up, and the steps to getting your data. Run your experiment and collect your data Enzyme Drops Used mL of juice produced Pectinase Cellulase Pectinase and Cellulase Which enzyme (or combination) appears to be more effective based off of juice produced? Graphing your Data Your teacher will give you direction here Conclusion, Evidence, Reasoning Which enzyme was most effective How do you know based on your data What is the science behind why it was so effective What is something you could do to improve this experiment if you were to run it again