Bacterial Transformation

Steps of Bacterial Transformation Put the steps in order. Step 1 Isolate gene for human insulin from a human pancreas cell Step 2 Remove a plasmid from a bacteria cell. Step 3 Cut the plasmid and human insulin gene with the same restriction enzyme creating sticky ends Step 4 Combine the plasmid and the human gene with ligase, creating a recombinant plasmid Step 5 Insert the recombinant plasmid into a bacteria cell. Step 6 The bacteria cell now has a working copy of the insulin gene and can produce human insulin. A ring of DNA in a bacterial cell is called recombinant DNA plasmid ligase Ligase is used for cutting the desired gene from the DNA cutting the plasmid open joining together the gene and plasmid A restriction enzyme is like scissors because it glues pieces of DNA together cuts DNA into desired segments Recombinant DNA is.... a piece of DNA that has been destroyed DNA that has been added to other DNA it normally wouldn't be in DNA that has grown through many cycles of cell division a piece of DNA that has been double checked for errors First you need to obtain a circular piece of DNA from bacteria which is called a plasmid . Then obtain a DNA sample that has the necessary gene . The plasmid and the DNA sample are both cut with the same restriction enzyme . The gene of interest is isolated and then incorporated into the plasmid using ligase . This plasmid is now recombinant DNA because it has DNA from two or more sources. Once the new DNA is placed in the bacteria cell the bacteria will express the desired trait .